Enzyme Handbook 16: First Supplement Part 2 Class 3: by Professor Dr. Dietmar Schomburg, Dr. Dörte Stephan (auth.),

By Professor Dr. Dietmar Schomburg, Dr. Dörte Stephan (auth.), Professor Dr. Dietmar Schomburg, Dr. Dörte Stephan (eds.)

The target of the Enzyme instruction manual is to supply in concise shape facts on enzymes sufficiently good characterised. the information sheets are prepared of their EC quantity series, volumes 15 to 17 include extra Enzymes and up-to-date info sheets to be inserted in earlier volumes by means of their EC-number. for every enzyme, systematic and customary names are given, info on response sort, substrate and product spectrum, inhibitors, cofactors, kinetic facts, pH and temperature variety, beginning, purification, molecular facts and garage stipulations are indexed. A reference record completes the knowledge sheets. This assortment is an critical resource of data for researchers utilising enzymes in research, synthe

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Extra info for Enzyme Handbook 16: First Supplement Part 2 Class 3: Hydrolases

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J. BioI. , Turk, V: BioI. Chem. : J. BioI. : BioI. Chem. , Turk, V: Eur. J. , Turk, V: BioI. Chem. 28 Systematic name Recommended name Picornain 3C Synonyms Picornavirus endopeptidase 3C Poliovirus protease 3C Poliovirus proteinase 3C Proteinase, poliovirus, 3C Rhinovirus protease 3C Proteinase, rhinovirus, 3C Foot-and-mouth protease 3C Proteinase, foot-and-mouth-disease virus, 3C Proteinase, picornavirus, 3C 3°C Protease 3°C Proteinase Coxsackievirus 3C proteinase Cysteine proteinase 3C Hepatitis A virus 3C proteinase Protease 3C Tomato ringspot nepovirus 3C-related protease More (not a homologue of papain) CAS Reg.

J. : J. : J. BioI. : J. BioI. , Fessl. : Proc. Natl. Acad. Sci. : Annu. Rev. 30 Systematic name Recommended name Caricain Synonyms Papaya peptidase A Papaya peptidase II Papaya proteinase OMEGA Papaya proteinase III Papaya proteinase 3 Proteinase omega Proteinase, papaya A Papaya proteinase A Proteinase, papaya, III Chymopapain S (1) PpOmega (14) More (problems of classification of papaya latex proteinases (2), names and numbers of papaya proteinases (4)) [2, 4) CAS Reg. No. 30 Substrate spectrum 1 Azocasein + H20 [6] 2 Albumin + Hp (low activity) [6] 3 Bovine nasal cartilage + Hp [6] 4 Succinyl-albumin + Hp [6] 5 Benzyloxycarbonyl-Phe-Arg 4-methylcoumarin 7-amide + H20 [6] 6 Benzyloxycarbonyl-Lys nitrophenyl ester + Hp [11, 12] 7 Casein + Hp (beta- [17], less active than papain) [9,17] 8 N-Benzoyl-L-Arg ethyl ester + H20 (less active than papain [9]) [7-9] 9 N-Tosyl-L-Arg methyl ester + Hp (less active than papain) [9] 10 N-Benzoyl-Arg amide + Hp (less active than papain) [9] 11 N-Benzoyl-Arg 4-nitroanilide + H2 0 (less active than papain [9]) [9, 11, 14,15,18] 12 Hippuric acid 4-nitrophenyl ester + Hp [12] 13 Benzyloxycarbonyl-Gly 4-nitrophenyl ester + Hp [12, 13] 14 Hide powder azure + H20 [17] Product spectrum 1 ?

2 3 4 5 6 7 8 9 10 11 12 13 14 ? ? ? ? ? ? ? 30 4 ISOLATION/PREPARATION Source organism Carica papaya (commercial chymopapain [8, 9]) [1-18) Source tissue Latex (non-fruit [3), fruit [S]) [1-18) Localization in source Purification Carica papaya (with monoclonal antibodies [10]) [10, 14) Crystallization Cloned Renatured 5 STABILITY pH Temperature (0C) More (denaturation occurs as a non two-state process, via an intermediate structure, thermodynamic characteristics) [18) Oxidation Organic solvent General stability information Storage 4°C, preparation of the mercury derivative of fully active papaya peptidase A, SO-fold molar excess of On.

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