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7: Imaging-setup consisting of a laser (L), the actual confocal microscope (M) and the imaging software (S). , Austria). To enable the SHG inducing of collagen, the OPO was tuned to 880 nm. For the detection of a backscattered second-harmonic signal, a bandpass (BP) 465/170 emission ﬁlter in combination with a nondescanned detector in epi-mode (backward detection) was used. 5 mm for deep tissue imaging (Schrieﬂ et al. , 2013). The resulting z-stacks comprised two-dimensional images that were recorded of the previously cleared heart specimen, and each z-stack contains sectional images with a range of vision of 620×620 μm in xy-plane.
The table includes the original position of the samples in the heart (speciﬁed by left ventricle (LV), endocardial (end), medial (med) and epicardial (epi)), the depth of the respective heart samples, as well as the computed rotation Φ per depth. The concentration parameter b, the coefﬁcient of determination R2 and the dispersion parameter κ were calculated for each image in the z-stack, the mean values of which are listed in the table for each of the 29 specimen. For better comparison of the rotation among the different samples, the rotation Φ was determined per mm depth for every sample.
In case of isotropic ﬁber distributions κ is 1/3 (b=0) (Schrieﬂ et al. , 2012). 1 Diffusion tensor imaging The feasibility study performed on a small part of a porcine heart wall led to the following results. 1 depicts the ﬁbers of the porcine heart sample in a variety of views, namely coronal (a), only the coronal plane is visible (b), sagittal (c), and axial (d) plane. 1, MA Institute of Technology, USA). 1: Visualization of a porcine heart sample following DTI, depicted in (a) coronal plane, (b) only the coronal plane is visible, (c) sagittal plane, and (d) axial plane.