Advances in Enzymology and Related Areas of Molecular by Alton Meister

By Alton Meister

Advances in Enzymology and similar components of Molecular Biology is a seminal sequence within the box of biochemistry, providing researchers entry to authoritative reports of the newest discoveries in all components of enzymology and molecular biology. those landmark volumes date again to 1941, delivering an unequalled view of the old improvement of enzymology. The sequence deals researchers the newest figuring out of enzymes, their mechanisms, reactions and evolution, roles in advanced organic technique, and their software in either the laboratory and undefined. every one quantity within the sequence positive aspects contributions by way of major pioneers and investigators within the box from world wide. All articles are rigorously edited to make sure thoroughness, caliber, and clarity.

With its wide variety of issues and lengthy historic pedigree, Advances in Enzymology and comparable components of Molecular Biology can be utilized not just by means of scholars and researchers in molecular biology, biochemistry, and enzymology, but in addition via any scientist drawn to the invention of an enzyme, its houses, and its applications.

Show description

Read Online or Download Advances in Enzymology and Related Areas of Molecular Biology, Volume 54 PDF

Best nonfiction_9 books

Glass-Ceramic Technology, Second Edition

Glass-ceramic fabrics proportion many homes with either glass and extra conventional crystalline ceramics. This new version examines some of the varieties of glass-ceramic fabrics, the equipment in their improvement, and their numerous purposes. With accelerated sections on biomaterials and hugely bioactive items (i.

The Protein Protocols Handbook

A amazing treasury of a hundred and forty four cutting-edge and hugely profitable analytical ideas for learning proteins and peptides. John Walker has integrated many up to date and more desirable equipment from his best-selling easy Protein and Peptide Protocols in addition to including approximately a hundred new ones. each one tried-and-tested protocol includes unique, step by step directions, time-saving troubleshooting assistance, substitute techniques, informative reasons, and entire lists of reagents and suppliers-aids now not frequently present in general magazine recipes.

Atherosclerosis: Experimental Methods and Protocols

Atherosclerosis-a significant killer of individuals in Western societies-is no longer but thoroughly understood. In Atherosclerosis: equipment and Protocols, Angela Drew and a panel of specialists have assembled a accomplished number of conventional and state-of-the-art ideas for investigating this illness and its attainable remedies.

Extra info for Advances in Enzymology and Related Areas of Molecular Biology, Volume 54

Example text

THE EXTENDED GLUTAMINE SUBSTRATE BINDING SITE Marked differences in substrate specificity between liver transglutaminase and factor XIIIa were recognized simultaneously with the identification of the factor as a transglutaminase (9,lO). E. FOLK ylglycine, which serve as effective substrates for the liver enzyme, are not acted on to any extent by factor XIIIa (10). In addition, examination of the patterns of crosslinking of human fibrin revealed that distinctly different products were formed with the liver enzyme and with factor XIIIa (9).

Residues 1-15 of the synthetic peptide correspond to residues 161-175 of p-casein (56). its interaction with the enzymes. First, determinants for recognition by both enzymes are contained in the linear sequence. Second, certain amino acid residues are more influential than others in directing the action of the enzymes. Third, the effects exerted by the amino acid residues are not the same with the two enzymes. Without going into the details of these data, it would seem that leucine 3, lysine 9 , valine 10, and leucine E-(7-GLUTAMYL) LYSINE BOND FORMATION 29 11 are important for factor XIIIa substrate interactions because the specificities for the peptides in which these residues are replaced by glycine are substantially lower than that for the model peptide.

An explanation for the forms in quotations of or-hydroxy-eaminocaproic acid and of its methyl ester is given in the text. The first substrate is the acetylated B chain of oxidized insulin. Reprinted with permission from reference 69. is true of their methyl esters, suggests a dominant role in the stereospecificity of the a-amino group of lysine and of the amido -NN-portion of peptide bonds involving this amino group. This suggestion gains strong support from the data plotted in Figure 11. Here specificities are for a number of compounds, each containing a single primary amino group and a single amide bond (carboxamides and sulfonamides).

Download PDF sample

Rated 4.99 of 5 – based on 13 votes