Advances In Biochemical Engineering Biotechnology by Bo Mattiasson, Lei Ye

By Bo Mattiasson, Lei Ye

Managed radical polymerization ideas for molecular imprinting, via Mark E. Byrne
From bulk polymers to nanoparticles, by way of Lei Ye
Post-imprinting and in-cavity functionalization, through Toshifumi Takeuchi
Characterization of MIPs (affinity, selectivity, web site heterogeneity…), via Richard Ansell
Theoretical points and computing device modelling, by way of Ian Nicholls
MIPs in aqueous environments, by means of Bin Lu
MIPs for binding macromolecules, by way of Kenneth J. Shea
Solid section extraction, by way of Ecevit Yilmaz
Sensors, by way of Sergey A. Piletsky
MIPs for catalysis and synthesis, through Marina Resmini
Wastewater remedy, by way of Bo Mattiasson
MIPs as instruments for bioassays, biotransformation and drug supply, by means of Meiping Zhao

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Amygdalus, have found biocatalytic applications for the production of (R)-cyanohydrins. The former of these Hnls is the least widely applied, the natural substrates being acetone cyanohydrin or (R)-2-butanone cyanohydrin (Table 1) [28]. Although an improved procedure for the purification of this enzyme has been reported [27] it is still only available in limited quantities (from 100 g of seedlings approximately 350 U of enzyme are obtained). It was found that this enzyme transforms a range of aliphatic aldehyde and ketone substrates [27], the latter of which included five-membered cyclic (e.

48 4 Enantiopure Cyanohydrins as Intermediates for the Synthesis of Bioactive Compounds . . . . . . . . . . . . 2 Chemical Transformations . . . . . . . . . . . . 49 Unsaturated Cyanohydrins . . . . . . . . . . . . 51 5 Conclusions and Outlook 6 References . . . . . . . . . . . . . . . . 53 . . . . . . . . . . . . g. biotransformations [1a–c, 2], will be very advantageous. In this respect, the hydroxynitrile lyases (Hnl) and their catalytic production of chiral cyanohydrins may have a significant role to play.

71 Advances in Biochemical Engineering / Biotechnology, Vol. 63 Managing Editor: Th. J. Carnell 1 Introduction Current regulations governing the introduction of chiral molecules as pharmaceuticals or agrochemicals require that each enantiomer should undergo testing for biological activity prior to development and marketing [1].

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